Caracterización energética de la unión de 1,3,6- naftalén trisulfonato al factor de crecimiento para fibroblastos ácido humano: implicaciones para su uso como agente anti-angiogénico

The equilibrium interaction of anti-cancer agent 1,3,6-naphatalene trisulfonate with human acidic fi broblast growthfactor has been studied by calorimetry. The affi nity decreases with increasing ionic strength. At pH 7.0 and 0.15 MNaCl concentration, a binding constant of the protein with the ligand was estimated in the 102 – 103 M-1 range, anaffi nity two orders of magnitude lower than that of aFGF with heparin. The interaction is enthalpically driven, andthe entropy change is unfavorable. A small heat capacity change with an unusual positive value of 90 cal K-1mol-1 wasdetermined from the temperature dependence of the enthalpies. Changes in accessible apolar and polar surface areasin the interaction were calculated from the thermodynamic data obtained and parametric equations in the literature.The results were compared with those measured from NMR data. The study includes structural bioenergetic considerationsabout the possible use of 1,3,6-naphatalene trisulfonate as an anti-angiogenic agent itself, or as a lead for thedevelopment of anti-angiogenic drugs.Se ha estudiado, mediante calorimetría isotérmica de reacción, la interacción del agente anticancerígeno 1,3,6-naftaléntrisulfonato con el factor de crecimiento para fi broblastos ácido humano. La afi nidad decrece con el aumento de lafuerza iónica. A pH 7,0 y NaCl 0,15 M, la constante de unión de la proteína con el ligando se encuentra en el rango102 – 103 M-1, una afi nidad dos órdenes de magnitud menor que la del FGFa por heparina. El cambio de entalpíafavorece la interacción, siendo el cambio de entropía desfavorable. De la dependencia del cambio de entalpía con latemperatura se calculó un pequeño cambio en la capacidad calorífi ca del proceso, con un valor excepcionalmentepositivo de 90 cal K-1mol-1. A partir de los datos termodinámicos medidos y de ecuaciones paramétricas establecidasen la literatura, se calcularon cambios en la superfi cie accesible al disolvente, tanto polar como apolar, que acompañana la interacción. Los resultados se compararon con los medidos mediante resonancia magnética nuclear. Elestudio incluye consideraciones de bioenergética estructural sobre el posible uso de 1,3,6-naftalén trisulfonato comoagente antiangiogénico o como molécula líder para el desarrollo de fármacos anti-angiogénicos

Use of life cycle assessment methodology in the analysis of ecological footprint assessment results to evaluate the environmental performance of universities

The assessment of the environmental performance of an organization is an essential part of the decision making process of an Environmental Management System. Having robust indicators enables a reliable assessment. The Ecological Footprint Assessment is used in different types of organizations, including universities. Its ability to clearly communicate over-consumption by using a land-base unit is an advantage when involving the university community in achieving better environmental performance. However, its lack of standardization makes it difficult to use as an indicator. It is believed that Life Cycle Assessment offers a framework with which to standardize the Ecological Footprint Assessment. In this paper, an Ecological Footprint Assessment considering Life Cycle Assessment methodology is developed as a case study for Universitat Politecnica de Valencia. Findings regarding the critical decisions of the methodology are compared with 23 Ecological Footprint Assessments of universities using a Life Cycle Assessment framework. Only 26% of the studies analyzed reference the Life Cycle Assessment methodology. Critical decisions such as defining a Functional Unit were relevant but not standardized, while the definition of the product system was the most standardized and homogeneous decision. The difficulty of gathering information when Environmental Management Systems are not available makes the Ecological Footprint Assessment a weak indicator. Nevertheless, results show that Life Cycle Assessment can guide an Ecological Footprint Assessment methodology where comparability and reliability is possible. (C) 2016 Elsevier Ltd. All rights reserved.Lo-Iacono-Ferreira, VG.; Torregrosa López, JI.; Capuz-Rizo, SF. (2016). Use of life cycle assessment methodology in the analysis of ecological footprint assessment results to evaluate the environmental performance of universities. Journal of Cleaner Production. 133:45-53. doi:10.1016/j.jclepro.2016.05.046S455313

Feel4Diabetes healthy diet score: Development and evaluation of clinical validity

Background: The aim of this paper is to present the development of the Feel4Diabetes Healthy Diet Score and to evaluate its clinical validity. Methods: Study population consisted of 3268 adults (63% women) from high diabetes risk families living in 6 European countries. Participants filled in questionnaires at baseline and after 1 year, reflecting the dietary goals of the Feel4Diabetes intervention. Based on these questions the Healthy Diet Score was constructed, consisting of the following components: breakfast, vegetables, fruit and berries, sugary drinks, whole-grain cereals, nuts and seeds, low-fat dairy products, oils and fats, red meat, sweet snacks, salty snacks, and family meals. Maximum score for each component was set based on its estimated relative importance regarding T2DM risk, higher score indicating better quality of diet. Clinical measurements included height, weight, waist circumference, heart rate, blood pressure, and fasting blood sampling, with analyses of glucose, total cholesterol, HDL-cholesterol, LDL-cholesterol, and triglycerides. Analysis of (co) variance was used to compare the Healthy Diet Score and its components between countries and sexes using baseline data, and to test differences in clinical characteristics between score categories, adjusted for age, sex and country. Pearson''s correlations were used to study the association between changes from baseline to year 1 in the Healthy Diet Score and clinical markers. To estimate reproducibility, Pearson''s correlations were studied between baseline and 1 year score, within the control group only. Results: The mean total score was 52.8 ± 12.8 among women and 46.6 ± 12.8 among men (p < 0.001). The total score and its components differed between countries. The change in the Healthy Diet Score was significantly correlated with changes in BMI, waist circumference, and total and LDL cholesterol. The Healthy Diet Score as well as its components at baseline were significantly correlated with the values at year 1, in the control group participants. Conclusion: The Feel4Diabetes Healthy Diet Score is a reproducible method to capture the dietary information collected with the Feel4Diabetes questionnaire and measure the level of and changes in the adherence to the dietary goals of the intervention. It gives a simple parameter that associates with clinical risk factors in a meaningful manner

Nurses' perceptions of aids and obstacles to the provision of optimal end of life care in ICU

Contains fulltext : 172380.pdf (publisher's version ) (Open Access

Energetic characterization of 1,3,6-naphtalene trisulfonate binding to human acidic fi broblast growth factor: implications for its anti-angiogenic use

Se ha estudiado, mediante calorimetría isotérmica de reacción, la interacción del agente anticancerígeno 1,3,6-naftalén trisulfonato con el factor de crecimiento para fi broblastos ácido humano. La afi nidad decrece con el aumento de la fuerza iónica. A pH 7,0 y NaCl 0,15 M, la constante de unión de la proteína con el ligando se encuentra en el rango 102 – 103 M-1, una afi nidad dos órdenes de magnitud menor que la del FGFa por heparina. El cambio de entalpía favorece la interacción, siendo el cambio de entropía desfavorable. De la dependencia del cambio de entalpía con la temperatura se calculó un pequeño cambio en la capacidad calorífi ca del proceso, con un valor excepcionalmente positivo de 90 cal K-1mol-1. A partir de los datos termodinámicos medidos y de ecuaciones paramétricas establecidas en la literatura, se calcularon cambios en la superfi cie accesible al disolvente, tanto polar como apolar, que acompañan a la interacción. Los resultados se compararon con los medidos mediante resonancia magnética nuclear. El estudio incluye consideraciones de bioenergética estructural sobre el posible uso de 1,3,6-naftalén trisulfonato como agente antiangiogénico o como molécula líder para el desarrollo de fármacos anti-angiogénicos.The equilibrium interaction of anti-cancer agent 1,3,6-naphatalene trisulfonate with human acidic fi broblast growth factor has been studied by calorimetry. The affi nity decreases with increasing ionic strength. At pH 7.0 and 0.15 M NaCl concentration, a binding constant of the protein with the ligand was estimated in the 102 – 103 M-1 range, an affi nity two orders of magnitude lower than that of aFGF with heparin. The interaction is enthalpically driven, and the entropy change is unfavorable. A small heat capacity change with an unusual positive value of 90 cal K-1mol-1 was determined from the temperature dependence of the enthalpies. Changes in accessible apolar and polar surface areas in the interaction were calculated from the thermodynamic data obtained and parametric equations in the literature. The results were compared with those measured from NMR data. The study includes structural bioenergetic considerations about the possible use of 1,3,6-naphatalene trisulfonate as an anti-angiogenic agent itself, or as a lead for the development of anti-angiogenic drugs

Treatment of squamous lung cell carcinoma with inhaled etamsylate

Aberrant expression of fibroblast growth factor and its receptors plays an important role in carcinogenesis. Here we report that local application of etamsylate, an inhibitor of fibroblast growth factor signaling pathways, led to a clinical improvement in a patient with squamous lung cell carcinoma